Marler has been retained as one of six remaining front-rowers in a squad of 25 to continue preparations for the showdown with the Wallabies, which means the Harlequins prop is certain to feature, either from the start or off the bench.
It follows his disciplinary reprieve last month, when he was initially banned for foul play until November 19, only to have the suspension end-date moved to the November 12 on appeal.
Meanwhile Harlequins prop Joe Marler will make his England comeback after being retained
Marler’s return will add experience to the England pack as the 27-year-old with 51 caps has been preferred to Leicester’s rookie loosehead Ellis Genge, who is among a group of nine players released back to their clubs. That group also contains Kruis.
Saracens’ Lions lock started in last Saturday’s 21-8 victory over Argentina but he will take no part in this second match of the Autumn campaign.
His release may be related to the England coaches’ desire to manage the work-load of their Lions contingent, but it may simply mean that he has been dropped, based on form.
Leicester’s rookie loosehead Ellis Genge is one of nine players released back to their clubs
Saracens duo Owen Farrell and Maro Itoje have been retained ahead of their expected return
Mike Brown will stay in camp but is unlikely to appear as he goes through concussion protocols
Owen Farrell and Maro Itoje were rested last weekend but retained in the squad in order to be around on match day.
Both men are on course to return to action against Australia, along with Jonny May – the prolific Leicester wing who has recovered from a hamstring injury which forced him to miss the encounter with the Pumas.
Along with Genge and Kruis, the other players who have been released back to their clubs are Tom Dunn, Piers Francis, Nick Isiekwe, Alex Lozowski, Zach Mercer, Marcus Smith and Denny Solomona.
Mike Brown will stay in camp, despite the Harlequins full-back being unlikely to appear on Saturday, as he goes through concussion protocols.
FULL 25-MAN SQUAD FOR AUSTRALIA TEST AT TWICKENHAM
Mike Brown (Harlequins), Elliot Daly (Wasps), Jonny May (Leicester Tigers), Semesa Rokoduguni (Bath Rugby), Anthony Watson (Bath Rugby)
Danny Care (Harlequins), Owen Farrell (Saracens), George Ford (Leicester Tigers), Jonathan Joseph (Bath Rugby), Henry Slade (Exeter Chiefs), Ben Youngs (Leicester Tigers)
Charlie Ewels (Bath Rugby), Nathan Hughes (Wasps), Maro Itoje (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Chris Robshaw (Harlequins), Sam Simmonds (Exeter Chiefs), Sam Underhill (Bath Rugby).
Dan Cole (Leicester Tigers), Jamie George (Saracens), Dylan Hartley (Northampton Saints), Joe Marler (Harlequins), Mako Vunipola (Saracens), Harry Williams (Exeter Chiefs).
PLAYERS RELEASED TO THEIR CLUBS
Tom Dunn (Bath Rugby), Piers Francis (Northampton Saints), Ellis Genge (Leicester Tigers), Nick Isiekwe (Saracens), George Kruis (Saracens), Alex Lozowski (Saracens), Zach Mercer (Bath Rugby), Marcus Smith (Harlequins), Denny Solomona (Sale Sharks).
*Ben Te’o (Worcester Warriors) will remain in camp until Wednesday